PXD036086-2
PXD036086 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | High-throughput analysis of ADME proteins in plasma extracellular vesicles for clinical surveillance |
Description | Clinical monitoring and prediction on drug ADME behaviors is of great essence due to the variability from individual patients. To date, pharmacogenomic approaches have allowed genotypic detection on a few metabolizing enzymes, mainly limited to cytochrome P450 polymorphisms. However, it is inadequate to hinge on genotype to sketch the whole real-time phenotype. Fortunately, the booming field of biofluid extracellular vesicles (EVs) offers an attractive option in clinical liquid biopsy. And the proteins inside EVs are able to reveal the phenotypes of parental cells from lesions or tissues, for example, EV metabolizing enzymes to reflect metabolism in liver. With liver tissue EV proteome as foundation, herein, we utilized a tandem mass tag-carrier strategy to characterize ADME proteins from plasma EVs. Mined from 44 identifiable and prevalent ADME proteins, 34 stringently quantified proteins emerged. As a result, previously-annotated mouse cytochrome P450 3A11 (Cyp3a11, homolog to human CYP3A4) and UDP glucuronosyltransferase 2A3 (Ugt2a3) were kinetically induced upon daily rifampicin dosage. Besides, dasatinib (Sprycel), a tyrosine kinase inhibitor to treat several subtypes of leukemia, was proved to timely elevate Cyp3a11 abundance in mouse plasma EVs, but less strong than rifampicin. Altogether, EV-based liquid biopsy is a promising direction to describe ADME activities in an interindividual manner. |
HostingRepository | jPOST |
AnnounceDate | 2022-08-17 |
AnnouncementXML | Submission_2022-09-18_02:34:04.399.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marco Hadisurya |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-16 11:33:05 | ID requested | |
1 | 2022-08-16 11:33:08 | announced | |
⏵ 2 | 2022-09-18 02:34:04 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: drug ADME | extracellular vesicles | proteomics | P450 enzymes | mass spectrometry |
Contact List
W. Andy Tao | |
---|---|
lab head | |
Marco Hadisurya | |
contact affiliation | Purdue University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001818/ |