PXD036060-1
PXD036060 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of SVEP1 protein interactions using affinity and proximity-based purifications coupled with mass spectrometry |
| Description | SVEP1 is a putative extracellular matrix (ECM) protein that promotes atherosclerosis in humans and mice. Antibodies that reliably recognize SVEP1 in situ have not been successfully developed. As a result, little is known about how and where SVEP1 may integrate into the ECM or which cell types it may influence. We used a combination of affinity and proximity-based experimental approaches to address this question. The bait proteins for these experiments included recombinant murine SVEP1 fused to a Myc-tag to allow for affinity-based co-immunoprecipitation or the promiscuous biotin ligase mini-Turbo ID to allow for proximity-based labeling and pulldown with streptavidin beads. The prey proteins were derived from enriched media of cultured murine VSMCs. Two independent experiments were performed using each approach and a reproducibility criterion of P < 0.10 for enrichment was applied across all experimental data. In addition to SVEP1, other ECM proteins that were identified include Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2, also known as Perlecan), Fibronectin, Laminin subunit gamma-1, and Nidogen-1. Together, these proteins comprise the major non-collagen basement membrane components. This suggests SVEP1 may be integrated into the basement membrane. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-01-09 |
| AnnouncementXML | Submission_2023-01-09_06:23:38.832.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jim Malone |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Acetyl; Deamidated; Gln->pyro-Glu; Oxidation; Pyro-carbamidomethyl |
| Instrument | Q Exactive Plus; timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-08-15 07:50:42 | ID requested | |
| ⏵ 1 | 2023-01-09 06:23:39 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: SVEP1, basement membrane |
Contact List
| Nathan Stitziel MD, PhD | |
|---|---|
| contact affiliation | Washington University School of Medicine |
| contact email | nstitziel@wustl.edu |
| lab head | |
| Jim Malone | |
| contact affiliation | Washington University Sch |
| contact email | jmalone@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090134/ |
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