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PXD036023-1

PXD036023 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Landscape of Human Spermatozoa: Optimized Extraction Method and Application
DescriptionHuman spermatozoa proteomics exposed to some physical, biological or chemical stressors is being explored. However, there is a lack of systematic comparative study on protein extraction methods to achieve in-depth coverage. Meanwhile, it is not clear if antibiotics can regulate proteins to affect sperm quality. Here we systematically compared and optimized the sperm protein extraction methods. It showed that the UA_Ultrasonication extraction method can make more kinds of protein to obtain higher protein extraction rate. Applying the optimized method, a largest human sperm proteome (5685 protein groups) from healthy human sperm samples was described. Besides, we continually investigated for the first time the differential sperm characteristics between medication (amoxicillin and clarithromycin) period and withdrawal period’s sperm samples obtained from a patient with Helicobacter pylori infection using semen examination, morphological analysis, bioinformatic analysis and data-independent acquisition tandem mass spectrometry (DIA-MS/MS). The longitudinal study result indicated that antibiotics can disrupt proteome expression in sperm, leading quantitative coverage of 159 differentially expressed proteins. The reliability of the results was verified by four proteins (ACTB, SCRN2, FDFT1, PLPP1) quantified by western blot. These proteins were mainly located at cytosol, involved in phosphorylation process, adenosine triphosphate (ATP) binding and metabolic pathways using bioinformatic analysis. This work provides an optimized extraction method to characterize the in-depth human sperm proteome and to extend its clinical applications. In addition, antibiotics should be avoided when it is necessary to preserve sperm quality for reproductive purpose.
HostingRepositoryPRIDE
AnnounceDate2022-12-18
AnnouncementXMLSubmission_2022-12-18_15:51:27.671.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYongZhang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-12 13:19:09ID requested
12022-12-18 15:51:28announced
22023-11-14 08:58:20announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Spermatozoa, Extraction method, Antibiotic, Mass spectrometry, Proteomics
Contact List
YongZhang
contact affiliationWest China Hospital,Sichuan University
contact emailnankai1989@foxmail.com
lab head
YongZhang
contact affiliationSichuan University
contact emailnankai1989@foxmail.com
dataset submitter
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Dataset FTP location
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