PXD035777 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Adaptor molecule of DNA methylation in IECs |
Description | We previously reported that gut microbiota induce de novo DNA methylation of the TLR4 gene in intestinal epithelial cells. Since DNA methyltransferase (DNMT) 3 mediates the transfer of methyl groups to any gene undergoing de novo methylation, the question of how gut microbiota induce the recruitment of DNMT3 to specific genes, including TLR4, remains to be addressed. In this study, we tried to identify an adaptor molecule that recruits DNMT3b to the TLR4 gene by comparing expression of DNMT3-interacting proteins between IEC lines with hypermethylated and hypomethylated TLR4 gene. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:45.093.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kyoko Takahashi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-02 11:07:11 | ID requested | |
1 | 2023-03-11 04:38:50 | announced | |
⏵ 2 | 2023-11-14 08:42:45 | announced | 2023-11-14: Updated project metadata. |
Publication List
Narabayashi H, Koma C, Nakata K, Ikegami M, Nakanishi Y, Ogihara J, Tsuda M, Hosono A, Hanazawa S, Takahashi K, Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions. Front Mol Biosci, 9():1005136(2022) [pubmed] |
Keyword List
submitter keyword: LC-MS/MS, Human intestinal epithelial cell lines |
Contact List
Kyoko Takahashi |
contact affiliation | Department of Applied Biological Science, College of Bioresource Sciences, Nihon University |
contact email | takahashi.kyoko@nihon-u.ac.jp |
lab head | |
Kyoko Takahashi |
contact affiliation | Nihon University |
contact email | takahashi.kyoko@nihon-u.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035777 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035777
- Label: PRIDE project
- Name: Adaptor molecule of DNA methylation in IECs