PXD035777

PXD035777 is an original dataset announced via ProteomeXchange.

Title	Adaptor molecule of DNA methylation in IECs
Description	We previously reported that gut microbiota induce de novo DNA methylation of the TLR4 gene in intestinal epithelial cells. Since DNA methyltransferase (DNMT) 3 mediates the transfer of methyl groups to any gene undergoing de novo methylation, the question of how gut microbiota induce the recruitment of DNMT3 to specific genes, including TLR4, remains to be addressed. In this study, we tried to identify an adaptor molecule that recruits DNMT3b to the TLR4 gene by comparing expression of DNMT3-interacting proteins between IEC lines with hypermethylated and hypomethylated TLR4 gene.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:45.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyoko Takahashi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 11:07:11	ID requested
1	2023-03-11 04:38:50	announced
⏵ 2	2023-11-14 08:42:45	announced	2023-11-14: Updated project metadata.

Narabayashi H, Koma C, Nakata K, Ikegami M, Nakanishi Y, Ogihara J, Tsuda M, Hosono A, Hanazawa S, Takahashi K, Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions. Front Mol Biosci, 9():1005136(2022) [pubmed]

submitter keyword: LC-MS/MS, Human intestinal epithelial cell lines

Kyoko Takahashi
contact affiliation	Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
contact email	takahashi.kyoko@nihon-u.ac.jp
lab head
Kyoko Takahashi
contact affiliation	Nihon University
contact email	takahashi.kyoko@nihon-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035777

PRIDE project URI

• PRIDE
  1. PXD035777
     1. Label: PRIDE project
     2. Name: Adaptor molecule of DNA methylation in IECs