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PXD035761-1

PXD035761 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of putative reader proteins of RNA 5-methylcytosine and its derivatives in Caenorhabditis elegans
DescriptionMethylation of carbon-5 of cytosines (m5C) is a conserved post-transcriptional nucleotide modification of RNA with widespread distribution across organisms. m5C has been shown to participate in mRNA transport and maintain mRNA stability through its recognition by the reader proteins ALYREF and YBX1, respectively. We recently showed that m5C is required for Caenorhabditis elegans development and fertility under heat stress. To contribute to the understanding of how m5C and its oxidative derivatives mediate their functions, we developed RNA baits bearing modified cytosines in diverse structural contexts to pulldown potential readers in C. elegans. Our mass spectrometry analyses reveal unique binding proteins for each of the modifications. We validate our dataset by demonstrating that the nematode ALYREF homologues ALY-1 and ALY-2 preferentially bind m5C in vitro. The dataset presented here serves as an important scientific resource that will support the discovery of new functions of m5C and its derivatives.
HostingRepositoryPRIDE
AnnounceDate2022-09-29
AnnouncementXMLSubmission_2022-09-29_09:00:53.775.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlejandro Brenes
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-02 10:47:05ID requested
12022-09-29 09:00:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Caenorhabditis elegans, c elegans, label free
Contact List
Angus I. Lamond
contact affiliationCentre for Gene Regulation and Expression
contact emaila.i.lamond@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliationUniversity of Dundee
contact emailajbrenesmurillo@dundee.ac.uk
dataset submitter
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Dataset FTP location
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