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PXD035622-1

PXD035622 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial interactome quantitation reveals structural changes in metabolic machinery in the failing murine heart
DescriptionAdvancements in cross-linking mass spectrometry (XL-MS) for structural analysis of proteins bridge the gap between purified systems and native tissue environments. Here, isobaric quantitative protein interaction reporter technology (iqPIR) was utilized to further extend XL-MS to the first system-wide comparative study of mitochondrial proteins from healthy and diseased murine hearts. The failing heart interactome includes 588 statistically significant cross-linked peptide pairs altered in the disease condition. Structural insight into ketone-oxidation metabolons, OXPHOS machinery, and nucleotide transporter hybrid-conformations support mitochondrial remodeling in failing hearts while bringing forth new hypotheses for pathological mechanisms. The application of quantitative cross-linking technology in tissue provides molecular-level insight into complex biological systems challenging to model in cell culture, thus providing a valuable resource for studying human diseases.
HostingRepositoryPRIDE
AnnounceDate2022-08-02
AnnouncementXMLSubmission_2022-08-02_01:58:25.994.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaoting Tang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-29 07:04:32ID requested
12022-08-02 01:58:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: quantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biology
Contact List
James E Bruce
contact affiliationDepartment of Genome Sciences, University of Washington, Seattle, WA, USA
contact emailjimbruce@uw.edu
lab head
Xiaoting Tang
contact affiliationUniversity of Washington
contact emailxiaoting@uw.edu
dataset submitter
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Dataset FTP location
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