PXD035499 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of MHC-I presented porcine respiratory and re-productive syndrome virus (PRRSV) peptides reveals immu-nogenic epitopes within several non-structural proteins recog-nized by CD8+ T cells |
Description | Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most relevant por-cine pathogens worldwide. Active control of the disease relies on modified live virus vaccines (MLVs), as most inactivated vaccines provide very limited protection. Neutralizing antibodies occur late in infection, therefore CD8+ T cells are considered important correlates of protection and are a frequent focus of investigation. Our aim was to identify viral peptides naturally bound by the class I major histocompatibility complex (MHC-I), and to confirm their ability to stimu-late CD8+ T cells. For this purpose, we immunoprecipitated MHC-I/peptide complexes of PRRSV (strain AUT15-33) -infected cells (SLA-I Lr-Hp 35.0/24mod) to isolate the viral epitopes and ana-lyzed them with liquid chromatography coupled to mass spectrometry (LC-MS). Furthermore, we employed these identified peptides to stimulate peripheral blood mononuclear cells (PBMCs) of previously PRRSV infected pigs and measured the PRRSV-specific CD8+ T cell re-sponse with an intracellular cytokine staining (ICS). Our data reveals that PRRSV non-structural proteins (NSPs) encoded in open reading frame 1a and 1b (ORF1) present the major source of MHC-I presented peptides. Additionally, we show that our identified epitopes are able to trig-ger IFNγ responses in vitro. These findings are a basis for understanding the proteasomal degra-dation of PRRSV proteins, the cellular ability to display them via MHC-I, and their potential to restimulate CD8+ T cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:20:27.598.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD035499 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marlene Mötz |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-21 07:59:30 | ID requested | |
1 | 2022-10-14 11:01:01 | announced | |
⏵ 2 | 2023-11-14 07:20:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD035499; |
M, ö, tz M, Stas MR, Hammer SE, Duckova T, Fontaine F, Kiesler A, Seitz K, Ladinig A, M, ü, ller AC, Riedel C, Saalm, ü, ller A, R, ü, menapf T, T Cells. Viruses, 14(9):(2022) [pubmed] |
Keyword List
submitter keyword: Porcine reproductive and respiratory syndrome virus |
PRRSV |
Arteriviridae |
CD8+ T cells |
im-munopeptidome |
epitopes |
swine leukocyte antigen class I |
major histocompatibility complex class I |
mass spectrometry |
restimulation |
intracellular cytokine staining |
Contact List
Till Rümenapf |
contact affiliation | Institue of Virology, University of Veterinary Medicine Vienna, Austria |
contact email | till.ruemenapf@vetmeduni.ac.at |
lab head | |
Marlene Mötz |
contact affiliation | Institute of Virology, University of Veterinary Medicine Vienna |
contact email | marlene.moetz@vetmeduni.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035499 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035499
- Label: PRIDE project
- Name: Identification of MHC-I presented porcine respiratory and re-productive syndrome virus (PRRSV) peptides reveals immu-nogenic epitopes within several non-structural proteins recog-nized by CD8+ T cells