PXD035499

PXD035499 is an original dataset announced via ProteomeXchange.

Title	Identification of MHC-I presented porcine respiratory and re-productive syndrome virus (PRRSV) peptides reveals immu-nogenic epitopes within several non-structural proteins recog-nized by CD8+ T cells
Description	Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most relevant por-cine pathogens worldwide. Active control of the disease relies on modified live virus vaccines (MLVs), as most inactivated vaccines provide very limited protection. Neutralizing antibodies occur late in infection, therefore CD8+ T cells are considered important correlates of protection and are a frequent focus of investigation. Our aim was to identify viral peptides naturally bound by the class I major histocompatibility complex (MHC-I), and to confirm their ability to stimu-late CD8+ T cells. For this purpose, we immunoprecipitated MHC-I/peptide complexes of PRRSV (strain AUT15-33) -infected cells (SLA-I Lr-Hp 35.0/24mod) to isolate the viral epitopes and ana-lyzed them with liquid chromatography coupled to mass spectrometry (LC-MS). Furthermore, we employed these identified peptides to stimulate peripheral blood mononuclear cells (PBMCs) of previously PRRSV infected pigs and measured the PRRSV-specific CD8+ T cell re-sponse with an intracellular cytokine staining (ICS). Our data reveals that PRRSV non-structural proteins (NSPs) encoded in open reading frame 1a and 1b (ORF1) present the major source of MHC-I presented peptides. Additionally, we show that our identified epitopes are able to trig-ger IFNγ responses in vitro. These findings are a basis for understanding the proteasomal degra-dation of PRRSV proteins, the cellular ability to display them via MHC-I, and their potential to restimulate CD8+ T cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:20:27.598.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035499
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marlene Mötz
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 07:59:30	ID requested
1	2022-10-14 11:01:01	announced
⏵ 2	2023-11-14 07:20:28	announced	2023-11-14: Updated project metadata.

10.6019/PXD035499;

M, ö, tz M, Stas MR, Hammer SE, Duckova T, Fontaine F, Kiesler A, Seitz K, Ladinig A, M, ü, ller AC, Riedel C, Saalm, ü, ller A, R, ü, menapf T, T Cells. Viruses, 14(9):(2022) [pubmed]

submitter keyword: Porcine reproductive and respiratory syndrome virus

PRRSV

Arteriviridae

CD8+ T cells

im-munopeptidome

epitopes

swine leukocyte antigen class I

major histocompatibility complex class I

mass spectrometry

restimulation

intracellular cytokine staining

Till Rümenapf
contact affiliation	Institue of Virology, University of Veterinary Medicine Vienna, Austria
contact email	till.ruemenapf@vetmeduni.ac.at
lab head
Marlene Mötz
contact affiliation	Institute of Virology, University of Veterinary Medicine Vienna
contact email	marlene.moetz@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035499

PRIDE project URI

• PRIDE
  1. PXD035499
     1. Label: PRIDE project
     2. Name: Identification of MHC-I presented porcine respiratory and re-productive syndrome virus (PRRSV) peptides reveals immu-nogenic epitopes within several non-structural proteins recog-nized by CD8+ T cells