PXD035444-1

PXD035444 is an original dataset announced via ProteomeXchange.

Title	The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo
Description	Endocytosis controls the perception of stimuli by modulating protein abundance at the plasma membrane. In plants, clathrin-mediated endocytosis is the most prominent internalization pathway and relies on two multimeric adaptor complexes, the AP-2 and the TPLATE complex (TPC). Ubiquitination is a well-established modification triggering endocytosis of cargo proteins, but how this modification is recognized to initiate the endocytic event remains elusive. Here, we show that TASH3, one of the large subunits of TPC, recognizes ubiquitinated cargo at the plasma membrane via its SH3 domain-containing appendage. TASH3 lacking this evolutionary specific appendage modification allows TPC formation, but the plants show severely reduced endocytic densities, which correlates with reduced endocytic flux. Moreover, comparative plasma membrane proteomics identified differential accumulation of multiple ubiquitinated cargo proteins for which we confirm altered trafficking. Our findings position TPC as a key player for ubiquitinated cargo internalization, allowing future identification of target proteins under specific stress conditions.
HostingRepository	PRIDE
AnnounceDate	2023-03-11
AnnouncementXML	Submission_2023-03-11_00:26:13.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DominiqueEeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 04:36:11	ID requested
⏵ 1	2023-03-11 00:26:14	announced
2	2023-11-14 08:39:22	announced	2023-11-14: Updated project metadata.

Grones P, De Meyer A, Pleskot R, Mylle E, Kraus M, Vandorpe M, Yperman K, Eeckhout D, Dragwidge JM, Jiang Q, Nolf J, Pavie B, De Jaeger G, De Rybel B, Van Damme D, The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo. Nat Plants, 8(12):1467-1483(2022) [pubmed]

submitter keyword: AP-MS,Arabidopsis, TPLATE complex, endocytosis

Daniel VanDamme
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
contact email	daniel.vandamme@psb.vib-ugent.be
lab head
DominiqueEeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035444

PRIDE project URI

• PRIDE
  1. PXD035444
     1. Label: PRIDE project
     2. Name: The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo