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PXD035312-1

PXD035312 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVitamin C modulates the levels of several proteins of the mitochondrial complex III and its activity in the mouse liver
DescriptionIn the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. Importantly, the levels of ascorbate found in the serum of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in microsomal enriched liver extracts (MEE) from our different ascorbate treated cohorts of Gulo-/- females and males. We found that the MS intensities of several proteins in the mitochondrial complex III of the electron transport chain correlated positively with liver ascorbate concentrations in both Gulo-/- females and males. Consequently, the mitochondrial complex III activity in Gulo-/- female and male mice treated with suboptimal hepatic concentrations of ascorbate was significantly lower than Gulo-/- mice treated with optimal ascorbate concentration. Finally, proteins involved in complement activation correlated negatively with liver ascorbate concentrations in both Gulo-/- males and females.
HostingRepositoryMassIVE
AnnounceDate2022-09-27
AnnouncementXMLSubmission_2022-09-27_08:58:42.831.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichel Lebel
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-13 10:55:36ID requested
12022-09-27 08:58:43announced
Publication List
no publication
Keyword List
submitter keyword: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, reactive oxygen species, sex-related differences, liver, mouse
Contact List
Michel Lebel
contact affiliationCentre Hospitalier Universite Laval
contact emailmichel.lebel@crchudequebec.ulaval.ca
lab head
Michel Lebel
contact affiliationCHudeQuebe
contact emailmichel.lebel@crchudequebec.ulaval.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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