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PXD035188-1

PXD035188 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInvestigation of the synergistic effect mechanism underlying sequential use of palbociclib and cisplatin through integral proteomic and glycoproteomic analysis
DescriptionChemoresistance largely hampers the clinical use of chemo drugs for cancer patients, combination or sequential drug treatment regimens have been designed to minimize chemotoxic and resensitize chemoresistance. In this work, the cytotoxic effect of cisplatin was found to be enhanced by palbociclib pretreatment. With the integration of proteomic and N-glycoproteomic workflow, we found that palbociclib alone mainly enhances the N-glycosylation alterations in Hela cells, while cisplatin majorly increases the DEPs (different expression proteins) including apoptosis proteins. As a result, the sequential use of two drugs induced a higher expression level of apoptosis proteins BAX and BAK. Those altered n-glycoproteins induced by palbociclib were implicated in pathways that are closely associated with cell membrane modification and drug sensitivity. Specifically, the top 4 frequently glycosylated proteins FOLR1, L1CAM, CD53, and LAMP1 were all drug resistance or drug sensitivity associated proteins located mainly in the cell membrane. It is suspected that palbociclib-induced N-glycosylation on the membrane protein allowed the Hela cell to become more vulnerable to cisplatin. Our study provided new insights into the mechanism underlying the sequential use of target drug and chemotherapy drug, meanwhile suggesting a high-efficiency approach that involves proteomic and N-glycoproteomic to facilitate drug understanding.
HostingRepositoryiProX
AnnounceDate2022-07-09
AnnouncementXMLSubmission_2022-07-08_23:11:35.568.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQian Xue
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-08 23:11:19ID requested
12022-07-08 23:11:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomics, Glycoproteomics, Palbociclib, Cisplatin, Chemoresistance
Contact List
Yang Zhao
contact affiliationNational Institute of Metrology, China
contact emailzynellj@126.com
lab head
Qian Xue
contact affiliationNational Institute of Metrology, China
contact emailnaiqeux@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI