PXD035184 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interactome of CFTR using Apex and TurboID proximity labelling |
Description | The proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches, enabling to better understand the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, several strategies have been developed based on the proximity labelling of protein partners or proteins in close vicinity and their subsequent identification by mass spectrometry. In this study, we evaluate TurboID and APEX2 proximity labelling approaches on WT CFTR and compare results to those obtained by co-immunoprecipitation and in databases. We then compared the CFTR-WT interactome to two mutants of CFTR (G551D and W1282X) and the structurally unrelated potassium channel KCNK3. The two methods efficiently identified both known and novel CFTR protein partners and in proximity such as multiple SLC transporters. Hence proximity labelling approaches enable to obtain a wider picture of the CFTR interactome, feeding databases and giving a better understanding of the CFTR environment. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:29:33.418.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chiara guerrera |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-08 05:43:55 | ID requested | |
1 | 2022-10-14 00:56:55 | announced | |
⏵ 2 | 2023-11-14 08:29:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Chevalier B, Baatallah N, Najm M, Castanier S, Jung V, Pranke I, Golec A, Stoven V, Marullo S, Antigny F, Guerrera IC, Sermet-Gaudelus I, Edelman A, Hinzpeter A, Differential CFTR-Interactome Proximity Labeling Procedures Identify Enrichment in Multiple SLC Transporters. Int J Mol Sci, 23(16):(2022) [pubmed] |
Keyword List
submitter keyword: Apex, TurboID, KCNK3,CFTR, interactome |
Contact List
Chiara Guerrera |
contact affiliation | INSERM US24/CNRS UAR3633, Proteomic Platform Necker, University of Paris Cité—Federative Research Structure Necker, Paris, 75015 France |
contact email | chiara.guerrera@inserm.fr |
lab head | |
Chiara guerrera |
contact affiliation | Necker proteomics, INSERM |
contact email | chiara.guerrera@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035184
- Label: PRIDE project
- Name: Interactome of CFTR using Apex and TurboID proximity labelling