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PXD035107-1

PXD035107 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal phosphoproteomic profiling of skeletal muscle in ovarian-hormone deficient mice
DescriptionPhosphorylation is important in skeletal muscle development, growth, regeneration, and contractile function. Alterations in the skeletal muscle phosphoproteome due to aging have been reported in males; however, studies in females are lacking. We have demonstrated that estrogen deficiency decreases muscle force which correlates with decreased myosin regulatory light chain phosphorylation. Thus, we questioned whether the decline of estrogen in females that occurs with aging might alter the skeletal muscle phosphoproteome. C57BL/6J female mice randomly assigned to a sham-operated (Sham) or ovariectomy (Ovx) group to investigate the effects of estrogen deficiency on skeletal muscle protein phosphorylation in a resting, non-contracting condition. After 16 weeks of estrogen deficiency, the tibialis anterior muscle was dissected and prepped for label-free nano-liquid chromatography tandem mass spectrometry phosphoproteomic analysis. We identified 4,780 phosphopeptides in tibialis anterior muscles of ovariectomized (Ovx) and Sham-operated (Sham) control mice. Further analysis revealed 647 differentially regulated phosphopeptides (Benjamini – Hochberg adjusted p-value < 0.05 and 1.5-fold change ratio) that corresponded to 130 proteins with 22 proteins differentially phosphorylated (3 unique to Ovx, two unique to Sham, six upregulated, and 11 downregulated). Differentially phosphorylated proteins associated with the sarcomere, cytoplasm, and metabolic and calcium signaling pathways were identified. Our work provides the first global phosphoproteomic analysis in females and how estrogen deficiency impacts the skeletal muscle phosphoproteome.
HostingRepositoryPRIDE
AnnounceDate2022-09-28
AnnouncementXMLSubmission_2022-09-28_13:29:37.869.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMina Peyton
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-06 14:11:02ID requested
12022-09-28 13:29:38announced
22023-11-14 08:56:44announced2023-11-14: Updated project metadata.
Publication List
10.1152/PHYSIOLGENOMICS.00104.2022;
Keyword List
submitter keyword: females, estrogen, ovariectomy, sarcomere, tropomyosin, obscurin, myosin heavy chain-2, myozenin-2, and calcium signaling
Contact List
Dawn A. Lowe
contact affiliationUniversity of Minnesota - Rehabilitation Science
contact emaillowex017@umn.edu
lab head
Mina Peyton
contact affiliationUniversity of MN
contact emailmina.peyton@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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