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PXD035078-2

PXD035078 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvaluation of absorbent cotton for glycopeptide enrichment
DescriptionThis study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the performance of cotton HILIC was evaluated by comparing with other two commercial enrichment approaches, venusil HILIC and Oasis MAX in both mouse brain and seminal plasma. Moreover, capacity and recovery rate were evaluated using different milligram of cotton in 200µL tips. Thus, a simple, convenient, and cost-friendly cotton HILIC method was proposed to achieve highly selective intact glycopeptide profiling.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:07.826.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiaomiao Xin
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcarbamoylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-04 14:58:55ID requested
12023-03-04 01:44:54announced
22023-11-14 08:56:08announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HILIC,Glycopeptide, Enrichment Absorbent cotton, Mass spectrometry, MAX
Contact List
Shisheng Sun
contact affiliationNorthwest University
contact emailsuns@nwu.edu.cn
lab head
Miaomiao Xin
contact affiliationCollege of Life Science, Northwest University, Xi’an, Shaanxi Province 710069, P. R. China
contact emailxinmiao1206@126.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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