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PXD035046-1

PXD035046 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRapid Monocyte Recruitment Dictates the Therapeutic Efficacy of Focal Radiotherapy
DescriptionThe recruitment of monocytes and their differentiation into immunosuppressive cells is associated with the negative outcome of non-conformal radiotherapy (RT). However, non-conformal RT is irrelevant in the clinic, and little is known about the role of monocytes following radiotherapy modes used in patients, such as conformal radiotherapy (CRT). Here, we investigated the acute immune infiltration post-CRT. Contrary to non-conformal RT approaches, we found that CRT induces a rapid and robust recruitment of monocytes to the tumor that minimally differentiate into tumor-associated macrophages (TAM) or dendritic cells (DC), but instead upregulate major histocompatibility complex II (MHCII) and costimulatory molecules. We establish that these large numbers of infiltrating monocytes are responsible for increasing type I interferon in the tumor microenvironment (TME), activation of CD8+ T cells and the reduction in tumor burden. Importantly, we demonstrate that rapid monocyte infiltration to the TME is hindered when RT inadvertently affects healthy tissues. Our results unravel a positive role of monocytes during clinically relevant modes of RT and demonstrate that limiting exposure of healthy tissues to radiation has a positive therapeutic effect on the overall immune response within the tumor.
HostingRepositoryMassIVE
AnnounceDate2023-05-03
AnnouncementXMLSubmission_2023-05-03_08:57:24.632.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuliana Idoyaga
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListTMTpro
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-30 07:37:20ID requested
12023-05-03 08:57:25announced
Publication List
no publication
Keyword List
submitter keyword: Radiation, Monocytes
Contact List
Juliana Idoyaga
contact affiliationStanford University
contact emailjidoyaga@stanford.edu
lab head
Juliana Idoyaga
contact affiliationStanford University
contact emailjidoyaga@stanford.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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