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PXD034986-1

PXD034986 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRNA-DNA hybrids prevent resection at dysfunctional telomeres
DescriptionAt critically short telomeres TERRA RNA-DNA hybrids become stabilized and drive homology-directed repair (HDR) to delay replicative senescence. However, even at long- and intermediate-length telomeres, not subject to HDR, transient TERRA RNA-DNA hybrids form, suggestive of additional roles. Here, we report that hybrids at telomeres prevent resection by the Exo1 nuclease when telomeres become non-functional. We employed the well-characterized cdc13-1 allele, where telomere resection can be induced in a temperature dependent manner, to demonstrate that ssDNA generation at telomeres is either prevented or augmented when RNA-DNA hybrids are stabilized or destabilized, respectively. The viability of cdc13-1 cells is affected by the presence or absence of hybrids accordingly. These results give insights into an additional role of TERRA at dysfunctional telomeres suggesting that it not only affects replicative senescence rates through HDR activation at critically short telomeres, but may also affect resection rates at intermediate length telomeres in pre-senescent cells.
HostingRepositoryPRIDE
AnnounceDate2023-01-18
AnnouncementXMLSubmission_2023-01-18_03:51:29.215.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFButter
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-30 02:55:59ID requested
12023-01-18 03:51:29announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MS/MS
Contact List
FalkButter
contact affiliationInstitute of Molecular Biology (IMB)
contact emailf.butter@imb.de
lab head
FButter
contact affiliationQuantitative Proteomics Institute of Molecular Biology (IMB)
contact emailf.butter@imb-mainz.de
dataset submitter
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Dataset FTP location
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