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PXD034970 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome analysis of Staphylococcus aureus strains
DescriptionCervimycins A‒D are natural products of Streptomyces tendae HKI 0179 with promising activity against multidrug resistant staphylococci and vancomycin resistant enterococci. To initiate mode of action studies, we selected cervimycin C and D resistant (CmR) Staphylococcus aureus strains. Genome sequencing of CmR mutants revealed amino acid exchanges in the essential histidine kinase WalK, the Clp protease proteolytic subunit ClpP or the Clp ATPase ClpC, and the heat shock protein DnaK. Proteomic analysis revealed massive alterations in CmR-02 (amino acid exchanges: ClpP-I29F, DnaK-A112P, WalK-A243V) compared to the parent strain S. aureus SG511 Berlin, with major modifications in the heat shock regulon, the metal ion homeostasis and the carbohydrate metabolism. These effects were alleviated in the antibiotic susceptible suppressor mutant 02REV (amino acid exchanges: ClpP-I29F/M31I, WalK-A243V/S191L).
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMirita Franz-Wachtel
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-29 15:30:10ID requested
12022-09-22 04:23:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: S. aureus, antibiotic, resistance, mutant, revertant, antibiotic treatment
Contact List
Gabriele Bierbaum
contact affiliationUniversity Hospital Bonn, Institute of Medical Microbiology, Immunology and Parasitology, Bonn, Germany
contact emailg.bierbaum@uni-bonn.de
lab head
Mirita Franz-Wachtel
contact affiliationProteome Center Tuebingen
contact emailmirita.franz@uni-tuebingen.de
dataset submitter
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Dataset FTP location
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