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PXD034964-1

PXD034964 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins that interact with TRAF6 in ADP-heptose- or IL-1β-stimulated HEK293 cells
DescriptionThe bacterial metabolite ADP-heptose activates the mammalian protein kinase ALPK1 (alpha protein kinase 1), which then phosphorylates TIFA (TRAF interacting protein with FHA domain). This leads to the oligomerisation of TIFA and the recruitment and oligomerisation of TRAF6 (TNF receptor-associated factor 6), activating a signalling network that culminates in the production of inflammatory mediators that mount responses to combat microbial infection. To identify other protein(s) that associate with TRAF6 in ADP-heptose-stimulated cells, we re-expressed FLAG-TRAF6 in TRAF6 KO cells, stimulated the cells with ADP-heptose and immunoprecipitated TRAF6 from the cell extracts using a FLAG antibody. Proteins associated with TRAF6 were identified by mass spectrometry and included the components of LUBAC (the linear ubiquitin assembly complex), the components of the TAK1 and IB kinase (IKK) complexes, TRAF2 and c-IAP1, providing insight into the molecular details and mode of operation of the ADP-heptose signalling pathway. In parallel experiments the cells were stimulated with IL-1b, a cytokine that also signals via TRAF6, which served as a control.
HostingRepositoryPRIDE
AnnounceDate2022-09-14
AnnouncementXMLSubmission_2022-09-14_01:36:36.959.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrederic Lamoliatte
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-29 15:07:20ID requested
12022-09-14 01:36:37announced
22023-11-14 08:57:35announced2023-11-14: Updated project metadata.
Publication List
10.1042/BCJ20220401;
Keyword List
submitter keyword: ALPK1, TIFA, ADP-heptose, TRAF6
Contact List
Professor Sir Philip Cohen
contact affiliationMRC Protein Phosphorylation and Ubiquitylation Unit, The Sir James Black Centre, University of Dundee, United Kingdom
contact emailp.cohen@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliationUniversity of Dundee
contact emailflamoliatte001@dundee.ac.uk
dataset submitter
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Dataset FTP location
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