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PXD034945-1

PXD034945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSmall changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity
DescriptionKinetochore protein phosphorylation promotes the correction of erroneous microtubule attachments to ensure faithful chromosome segregation during cell division. Determining how phosphorylation executes error correction requires an understanding of whether kinetochore substrates are completely (i.e. all-or-none) or only fractionally phosphorylated. Using quantitative mass spectrometry (MS), we measured phospho-occupancy on the conserved kinetochore protein Hec1 (NDC80) that directly binds microtubules. None of the positions measured exceeded ~50% phospho-occupancy, and the cumulative phospho-occupancy changed by only ~20% in response to changes in microtubule attachment status. The narrow dynamic range of phospho-occupancy is maintained, in part, by ongoing phosphatase activity. Further, both Cdk1-Cyclin B1 and Aurora kinases phosphorylate Hec1 to enhance error correction in response to different types of microtubule attachment errors. The low inherent phospho-occupancy promotes microtubule attachment to kinetochores while the high sensitivity of kinetochore-microtubule attachments to small changes in phospho-occupancy drives error correction and ensures high mitotic fidelity.
HostingRepositoryMassIVE
AnnounceDate2022-07-20
AnnouncementXMLSubmission_2022-07-20_03:36:10.762.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMark Adamo
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation; Dimethyl; Dimethyl:2H(6)13C(2); Phospho
InstrumentQ Exactive Plus; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-27 11:59:04ID requested
12022-07-20 03:36:11announced
Publication List
no publication
Keyword List
submitter keyword: kinetochore, phosphorylation, microtubule, chromosome segregation, mitosis, phospho-occupancy, Hec1, NDC80, Cdk1, Cyclin B1, Aurora
Contact List
Scott Gerber
contact affiliationThe Geisel School of Medicine at Dartmouth
contact emailscott.a.gerber@dartmouth.edu
lab head
Mark Adamo
contact affiliationDartmouth College
contact emailmark.e.adamo@dartmouth.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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