PXD034945
PXD034945 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity |
| Description | Kinetochore protein phosphorylation promotes the correction of erroneous microtubule attachments to ensure faithful chromosome segregation during cell division. Determining how phosphorylation executes error correction requires an understanding of whether kinetochore substrates are completely (i.e. all-or-none) or only fractionally phosphorylated. Using quantitative mass spectrometry (MS), we measured phospho-occupancy on the conserved kinetochore protein Hec1 (NDC80) that directly binds microtubules. None of the positions measured exceeded ~50% phospho-occupancy, and the cumulative phospho-occupancy changed by only ~20% in response to changes in microtubule attachment status. The narrow dynamic range of phospho-occupancy is maintained, in part, by ongoing phosphatase activity. Further, both Cdk1-Cyclin B1 and Aurora kinases phosphorylate Hec1 to enhance error correction in response to different types of microtubule attachment errors. The low inherent phospho-occupancy promotes microtubule attachment to kinetochores while the high sensitivity of kinetochore-microtubule attachments to small changes in phospho-occupancy drives error correction and ensures high mitotic fidelity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-07-20 |
| AnnouncementXML | Submission_2022-07-20_03:36:10.762.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mark Adamo |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Dimethyl; Dimethyl:2H(6)13C(2); Phospho |
| Instrument | Q Exactive Plus; Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-27 11:59:04 | ID requested | |
| ⏵ 1 | 2022-07-20 03:36:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: kinetochore, phosphorylation, microtubule, chromosome segregation, mitosis, phospho-occupancy, Hec1, NDC80, Cdk1, Cyclin B1, Aurora |
Contact List
| Scott Gerber | |
|---|---|
| contact affiliation | The Geisel School of Medicine at Dartmouth |
| contact email | scott.a.gerber@dartmouth.edu |
| lab head | |
| Mark Adamo | |
| contact affiliation | Dartmouth College |
| contact email | mark.e.adamo@dartmouth.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089738/ |
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