PXD034579 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of the proteome of human cytochrome c oxidase assembly factors - Experiment C |
Description | Mitochondrial cytochrome c oxidase (CcO) or respiratory chain complex IV is a heme aa3-copper oxygen reductase containing metal centers essential for holo-complex biogenesis and enzymatic function that are assembled by subunit-specific metallochaperones. The enzyme has two copper sites located in the catalytic core subunits. The COX1 subunit harbors the CuB site that tightly associates with heme a3 while the COX2 subunit contains the binuclear CuA site. Here, we report that in human cells the CcO copper chaperones form macromolecular assemblies and cooperate with several twin CX9C proteins to control heme a biosynthesis and coordinate copper transfer sequentially to the CuA and CuB sites. These data on CcO illustrate a mechanism that regulates the biogenesis of macromolecular enzymatic assemblies with several catalytic metal redox centers and prevents the accumulation of cytotoxic reactive assembly intermediates. We characterized the proteome of COX11, COX19 and PET191 by AP + LC-MS-MS |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-11_23:51:51.455.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034579 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Antonio Barrientos |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-16 02:40:41 | ID requested | |
⏵ 1 | 2022-08-11 23:51:52 | announced | |
Publication List
N, ý, vltov, á E, Dietz JV, Seravalli J, Khalimonchuk O, Barrientos A, Coordination of metal center biogenesis in human cytochrome c oxidase. Nat Commun, 13(1):3615(2022) [pubmed] |
Keyword List
submitter keyword: mitochondria, cytochrome c oxidase, COX11, COX19, PET191 |
Contact List
Antoni Barrientos |
contact affiliation | University of Miami School of Medicine |
contact email | abarrientos@med.miami.edu |
lab head | |
Antonio Barrientos |
contact affiliation | University of Miami School of Medicine |
contact email | abarrientos@med.miami.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034579
- Label: PRIDE project
- Name: Characterization of the proteome of human cytochrome c oxidase assembly factors - Experiment C