PXD034554 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of proteins interacting with KaeA, the subunit of KEOPS complex in Aspergillus nidulans in vivo. |
Description | The Kae1/KaeA protein is a subunit of the highly evolutionarily conserved KEOPS complex (Kinase, Endopeptidase and Other Proteins of Small size). The Kae1/KaeA gene has been identified in all previously sequenced genomes of Archea, Bacteria and Eucaryota, except for the most reduced genomes. The KEOPS complex participates in the universal modification of the tRNA converting adenosine A37 to N6-threonylcarbamoyl adenosine (t6A37). This modification of A37, immediately 3’ of anticodons decoding ANN codons, is one of the few tRNA modifications present in all organisms and plays an extremely important role in the accuracy of the translation process. We characterized two mutations in kaeA of filamentous fungus Aspergillus nidulans that result in the derepression of arginine catabolic enzymes and also showed that kaeA is involved in the regulation of several other genes that control various domains of cellular metabolism. This suggests that kaeA has additional function apart from t6A37 modification. To support this hypothesis we looked for proteins interacting with KaeA. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:28:54.557.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034554 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Emericella nidulans (Aspergillus nidulans); NCBI TaxID: 162425; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-16 01:47:02 | ID requested | |
1 | 2023-03-10 19:11:08 | announced | |
⏵ 2 | 2023-11-14 08:28:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Gawlik J, Koper M, Bogdanowicz A, Weglenski P, Dzikowska A, . Int J Mol Sci, 23(19):(2022) [pubmed] |
10.6019/PXD034554; |
Keyword List
submitter keyword: Aspergillus nidulans, interactomics |
Contact List
Agnieszka Dzikowska |
contact affiliation | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02–106 Warsaw, Poland |
contact email | adzik@igib.uw.edu.pl |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034554
- Label: PRIDE project
- Name: Identification of proteins interacting with KaeA, the subunit of KEOPS complex in Aspergillus nidulans in vivo.