PXD034554

PXD034554 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins interacting with KaeA, the subunit of KEOPS complex in Aspergillus nidulans in vivo.
Description	The Kae1/KaeA protein is a subunit of the highly evolutionarily conserved KEOPS complex (Kinase, Endopeptidase and Other Proteins of Small size). The Kae1/KaeA gene has been identified in all previously sequenced genomes of Archea, Bacteria and Eucaryota, except for the most reduced genomes. The KEOPS complex participates in the universal modification of the tRNA converting adenosine A37 to N6-threonylcarbamoyl adenosine (t6A37). This modification of A37, immediately 3’ of anticodons decoding ANN codons, is one of the few tRNA modifications present in all organisms and plays an extremely important role in the accuracy of the translation process. We characterized two mutations in kaeA of filamentous fungus Aspergillus nidulans that result in the derepression of arginine catabolic enzymes and also showed that kaeA is involved in the regulation of several other genes that control various domains of cellular metabolism. This suggests that kaeA has additional function apart from t6A37 modification. To support this hypothesis we looked for proteins interacting with KaeA.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:54.557.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034554
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Emericella nidulans (Aspergillus nidulans); NCBI TaxID: 162425;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 01:47:02	ID requested
1	2023-03-10 19:11:08	announced
⏵ 2	2023-11-14 08:28:55	announced	2023-11-14: Updated project metadata.

Gawlik J, Koper M, Bogdanowicz A, Weglenski P, Dzikowska A, . Int J Mol Sci, 23(19):(2022) [pubmed]

10.6019/PXD034554;

submitter keyword: Aspergillus nidulans, interactomics

Agnieszka Dzikowska
contact affiliation	Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02–106 Warsaw, Poland
contact email	adzik@igib.uw.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034554

PRIDE project URI

• PRIDE
  1. PXD034554
     1. Label: PRIDE project
     2. Name: Identification of proteins interacting with KaeA, the subunit of KEOPS complex in Aspergillus nidulans in vivo.