PXD034488

PXD034488 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of Chromochloris zofingiensis reveals key protein involved in cell growth and bioactive compound biosynthesis
Description	Glucose metabolism regulates cell growth and affects astaxanthin accumulation in green algae Chromochloris zofingiensis. Hub genes function in this bioactive compound biosynthesis has been illustrated in genome, transcriptome and metabolome level, but rather limited from proteome aspects. Microalgal cell produce an enhanced biomass (8-fold higher) but decreased lipid and astaxanthin content (~20% less) in glucose condition compared to the control. Here, we investigate the proteomic response of C. zofingiensis grown with vs without glucose using LC-MS/MS based Tandem Mass Tag (TMT) approach. Correspondingly, proteomic analysis demonstrated that glucose supplement triggers the up-regulation of 105 proteins and down-regulation of 151 proteins. Thus, the carbon and energy flux might flow to cell growth that increased associated proteins abundance including DNA polymerase, translation initiation factor, 26S proteasome regulatory subunits as well as the marker enzyme of TCA cycle ribosomal protein. Moreover, glucose supplement triggers the down-regulation of proteins mainly involved in photosynthesis, chloroplast, valine, leucine and isoleucine biosynthesis, 2-oxocarboxylic acid metabolism and pantothenate and CoA biosynthesis pathways. This proteomic analysis is likely to provide new insights about algal growth, lipid or astaxanthin accumulation upon glucose supplements, providing a foundation for further development of C. zofingiensis as oleaginous microalga in bioengineering respects.
HostingRepository	iProX
AnnounceDate	2022-06-13
AnnouncementXML	Submission_2022-09-27_20:56:55.863.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinshuai Sun
SpeciesList	scientific name: Chromochloris zofingiensis; NCBI TaxID: 31302;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-13 01:18:28	ID requested
1	2022-06-13 01:18:40	announced
⏵ 2	2022-09-27 20:56:56	announced	2022-09-28: Update publication information.

Qiu W, Chen R, Wang X, Liu J, Lv W, Reveals the Key Proteins Involved in Cell Growth and Bioactive Compound Biosynthesis. Plants (Basel), 11(14):(2022) [pubmed]

submitter keyword: Microalgae

Jinshuai Sun
contact affiliation	National Center for Occupational Safety and Health, NHC, Beijing
contact email	sjsshuai@126.com
lab head
Jinshuai Sun
contact affiliation	National Center for Occupational Safety and Health, NHC, Beijing
contact email	sjsshuai@126.com
dataset submitter

iProX dataset URI