Update publication information. Glucose metabolism regulates cell growth and affects astaxanthin accumulation in green algae Chromochloris zofingiensis. Hub genes function in this bioactive compound biosynthesis has been illustrated in genome, transcriptome and metabolome level, but rather limited from proteome aspects. Microalgal cell produce an enhanced biomass (8-fold higher) but decreased lipid and astaxanthin content (~20% less) in glucose condition compared to the control. Here, we investigate the proteomic response of C. zofingiensis grown with vs without glucose using LC-MS/MS based Tandem Mass Tag (TMT) approach. Correspondingly, proteomic analysis demonstrated that glucose supplement triggers the up-regulation of 105 proteins and down-regulation of 151 proteins. Thus, the carbon and energy flux might flow to cell growth that increased associated proteins abundance including DNA polymerase, translation initiation factor, 26S proteasome regulatory subunits as well as the marker enzyme of TCA cycle ribosomal protein. Moreover, glucose supplement triggers the down-regulation of proteins mainly involved in photosynthesis, chloroplast, valine, leucine and isoleucine biosynthesis, 2-oxocarboxylic acid metabolism and pantothenate and CoA biosynthesis pathways. This proteomic analysis is likely to provide new insights about algal growth, lipid or astaxanthin accumulation upon glucose supplements, providing a foundation for further development of C. zofingiensis as oleaginous microalga in bioengineering respects.