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PXD034448-2

PXD034448 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components
DescriptionThe establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we developed an accurate mass spectrometry (MS)-based proteomic method called Chromatin Aggregation Capture (ChAC) followed by Data-Independent Acquisition (DIA) and analyzed chromatome reorganizations during major phases of pluripotency. This enabled us to generate a comprehensive atlas of proteomes, chromatomes, and chromatin affinities for the ground, formative and primed pluripotency states, and to pinpoint the specific binding and rearrangement of regulatory components. These comprehensive datasets combined with extensive analyses identified phase-specific factors like QSER1 and JADE1/2/3 and provide a detailed foundation for an in-depth understanding of mechanisms that govern the phased progression of pluripotency. The technical advances reported here can be readily applied to other models in development and disease.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:50.453.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEnes Ugur
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-09 15:28:43ID requested
12023-02-27 06:13:57announced
22023-11-14 08:56:50announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: naive, DIA, pluripotency, mass spectrometry, stem cells, formative,Chromatin proteomics, primed
Contact List
Heinrich Leonhardt
contact affiliationFaculty of Biology and Center for Molecular Biosystems (BioSysM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München, Munich, 81377, Germany
contact emailh.leonhardt@lmu.de
lab head
Enes Ugur
contact affiliationFaculty of Biology and Center for Molecular Biosystems (BioSysM), Human Biology and BioImaging, LMU Munich & Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry
contact emailugur@bio.lmu.de
dataset submitter
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Dataset FTP location
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