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PXD034273-1

PXD034273 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803
DescriptionThe FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This protocol describes the acquisition of intact and active ATPase with good purity from Synechocystis sp. PCC 6803, based on a 3xFLAG tag fused to the beta subunit. The reproducibility of this protocol is high.
HostingRepositoryMassIVE
AnnounceDate2022-07-19
AnnouncementXMLSubmission_2022-07-19_02:18:20.087.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStefan Tholen
SpeciesList scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-03 03:14:27ID requested
12022-07-19 02:18:20announced
Publication List
no publication
Keyword List
submitter keyword: ATPase, Synechocystis, cyanobacteria
Contact List
Wolfgang R. Hess
contact affiliationGenetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg
contact emailwolfgang.hess@biologie.uni-freiburg.de
lab head
Stefan Tholen
contact affiliationUniklinik Freiburg
contact emailstefan.tholen@uniklinik-freiburg.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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