PXD034273

PXD034273 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803
Description	The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This protocol describes the acquisition of intact and active ATPase with good purity from Synechocystis sp. PCC 6803, based on a 3xFLAG tag fused to the beta subunit. The reproducibility of this protocol is high.
HostingRepository	MassIVE
AnnounceDate	2022-07-19
AnnouncementXML	Submission_2022-07-19_02:18:20.087.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Tholen
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-03 03:14:27	ID requested
⏵ 1	2022-07-19 02:18:20	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: ATPase, Synechocystis, cyanobacteria

submitter keyword: ATPase, Synechocystis, cyanobacteria

Contact List

Wolfgang R. Hess
contact affiliation	Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg
contact email	wolfgang.hess@biologie.uni-freiburg.de
lab head
Stefan Tholen
contact affiliation	Uniklinik Freiburg
contact email	stefan.tholen@uniklinik-freiburg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089593/