PXD034222-1

PXD034222 is an original dataset announced via ProteomeXchange.

Title	Oenothera effect on senescent NDHF
Description	Accumulation of senescent dermal fibroblasts drives skin aging, impairing the integrity of the extracellular matrix (ECM) and the function of neighbouring cells. One of strategies to manipulate cell senescence include the reactivation of proliferation. Here, a data independent mass spectrometry based proteomic approach has been used to evaluate the effect of an hydrophilic Oenothera biennis cell culture extract (ObHEx) on senescent human dermal fibroblasts. It has been shown that the extract was able to affect the levels of 18 proteins which cluster together and point to mitosis pathways. Indeed the treatment with ObHEx increase the expression of CDK1, replicative helicase complex (MCM2, MCM3, MCM4, MCM5, MCM6, MCM7), condensin I complex (NCAPD2, NCAPH, NCAPG, SMC4, SMC2) and other proteins related to kinetochore (KNTC1, NUF2, TRIP13). This strongly suggests that ObHEx could restore the proliferation of senescent fibroblasts.
HostingRepository	PRIDE
AnnounceDate	2023-03-11
AnnouncementXML	Submission_2023-03-10_19:13:14.650.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CerinaChhuon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-30 11:45:35	ID requested
⏵ 1	2023-03-10 19:13:15	announced
2	2023-11-14 08:28:59	announced	2023-11-14: Updated project metadata.

Ceccacci S, Roger K, Metatla I, Chhuon C, Tighanimine K, Fumagalli S, De Lucia A, Pranke I, Cordier C, Monti MC, Guerrera IC, on Senescent Human Dermal Fibroblasts. Int J Mol Sci, 23(23):(2022) [pubmed]

submitter keyword: hydrophilic extract of Oenothera biennis cell cultures

senescence

proteomics

data independent mass spectrometry

fibroblasts

Ida ChiaraGuerrera
contact affiliation	Head of the Necker Proteomics Plataform Paris France
contact email	chiara.guerrera@inserm.fr
lab head
CerinaChhuon
contact affiliation	Proteomics Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker, Université Paris Descartes, Paris, France
contact email	cerina.chhuon@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034222

PRIDE project URI

• PRIDE
  1. PXD034222
     1. Label: PRIDE project
     2. Name: Oenothera effect on senescent NDHF