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PXD034170-1

PXD034170 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBioprospecting the American Alligator Peptidome for Antiviral Peptides Against Venezuelan Equine Encephalitis Virus
DescriptionThe innate immune protection provided by cationic antimicrobial peptides (CAMPs) has been shown to extend to antiviral activity, with putative mechanisms of action including direct interaction with host cells or pathogen membranes. The lack of therapeutics available for the treatment of viruses such as Venezuelan equine encephalitis virus (VEEV) underscores the urgency of novel strategies for antiviral discovery. American alligator plasma has been shown to exhibit strong in vitro antibacterial activity, and functionalized hydrogel particles have been successfully employed for the identification of specific CAMPs from alligator plasma. Here, a novel bait strategy in which particles were encapsulated in membranes from either healthy or VEEV-infected cells was implemented to identify peptides preferentially targeting infected cells for subsequent evaluation of antiviral activity. Statistical analysis of peptide identification results was used to select five candidate peptides for testing, of which three exhibited a dose-dependent inhibition of VEEV, with one peptide also significantly inhibiting infectious titers. Results suggest our bioprospecting strategy provides a versatile platform that may be adapted for antiviral peptide identification from complex biological samples.
HostingRepositoryMassIVE
AnnounceDate2022-12-14
AnnouncementXMLSubmission_2022-12-14_10:37:37.806.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAmy Carfagno
SpeciesList scientific name: Alligator mississippiensis; common name: American alligator; NCBI TaxID: 8496;
ModificationListOxidation; Acetyl; Amidated
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-27 12:29:56ID requested
12022-12-14 10:37:38announced
Publication List
no publication
Keyword List
submitter keyword: antiviral peptides, membrane-encapsulated particles, non-model organism, Venezuelan equine encephalitis virus
Contact List
Barney Bishop
contact affiliationGeorge Mason University
contact emailbbishop1@gmu.edu
lab head
Amy Carfagno
contact affiliationGeorge Mason University
contact emailacarfagn@gmu.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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