PXD034170

PXD034170 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Bioprospecting the American Alligator Peptidome for Antiviral Peptides Against Venezuelan Equine Encephalitis Virus
Description	The innate immune protection provided by cationic antimicrobial peptides (CAMPs) has been shown to extend to antiviral activity, with putative mechanisms of action including direct interaction with host cells or pathogen membranes. The lack of therapeutics available for the treatment of viruses such as Venezuelan equine encephalitis virus (VEEV) underscores the urgency of novel strategies for antiviral discovery. American alligator plasma has been shown to exhibit strong in vitro antibacterial activity, and functionalized hydrogel particles have been successfully employed for the identification of specific CAMPs from alligator plasma. Here, a novel bait strategy in which particles were encapsulated in membranes from either healthy or VEEV-infected cells was implemented to identify peptides preferentially targeting infected cells for subsequent evaluation of antiviral activity. Statistical analysis of peptide identification results was used to select five candidate peptides for testing, of which three exhibited a dose-dependent inhibition of VEEV, with one peptide also significantly inhibiting infectious titers. Results suggest our bioprospecting strategy provides a versatile platform that may be adapted for antiviral peptide identification from complex biological samples.
HostingRepository	MassIVE
AnnounceDate	2022-12-14
AnnouncementXML	Submission_2022-12-14_10:37:37.806.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Amy Carfagno
SpeciesList	scientific name: Alligator mississippiensis; common name: American alligator; NCBI TaxID: 8496;
ModificationList	Oxidation; Acetyl; Amidated
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-27 12:29:56	ID requested
⏵ 1	2022-12-14 10:37:38	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: antiviral peptides, membrane-encapsulated particles, non-model organism, Venezuelan equine encephalitis virus

submitter keyword: antiviral peptides, membrane-encapsulated particles, non-model organism, Venezuelan equine encephalitis virus

Contact List

Barney Bishop
contact affiliation	George Mason University
contact email	bbishop1@gmu.edu
lab head
Amy Carfagno
contact affiliation	George Mason University
contact email	acarfagn@gmu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089560/