PXD034143 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of mouse Paneth cells microdissected from small intestinal tissue |
Description | Paneth cells are antimicrobial peptide-secreting cells located at the base of the crypts of the small intestine. The proteome of Paneth cells is not well defined because of their co-existence with stem cells making it difficult to culture Panth cells alone in vitro. Using a simplied toluidine blue O method for staining mouse intestinal tissue, laser capture microdissection (LCM) to isolate cells from the crypt region and surfactant assisted one pot protein digestion, we identified more than 1,300 proteins from crypts equivalent to 18,000 cells. Compared with the proteomes of villi and smooth muscle regions, the crypt proteome is highly enriched in defensins, lysozymes and other antimicrobial peptides that are characteristic of Paneth cells. The sensitivity of the LCM-based proteomics approach was also assessed using a smaller number of cell equivalent tissues, a comparable proteomic coverage can be achieved with 3,600 cells. This work is the first proteomics study of intestinal tissue enriched with Paneth cells. The simplied workflow enables profiling of Paneth cell associated pathological changes at the proteome level directly from frozen intestinal tissue. It may also be useful for proteomics studies of other spatially resolved cell types from other tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-12 |
AnnouncementXML | Submission_2022-10-12_07:10:29.644.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JongminWoo |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-26 01:32:33 | ID requested | |
⏵ 1 | 2022-10-12 07:10:30 | announced | |
2 | 2023-11-14 08:30:52 | announced | 2023-11-14: Updated project metadata. |
Publication List
Woo J, Schoenfeld M, Sun X, Iraguha T, Zhou Z, Zhang Q, Mouse Paneth Cell-Enriched Proteome Enabled by Laser Capture Microdissection. J Proteome Res, 21(10):2435-2442(2022) [pubmed] |
Keyword List
submitter keyword: Paneth cell, mouse small intestine, proteomics, laser capture microdissection, toluidine blue, villi |
Contact List
QibinZhang |
contact affiliation | 1. Center for Translational Biomedical Research, University of North Carolina at Greensboro 2. Department of Chemistry & Biochemistry, University of North Carolina at Greensboro |
contact email | q_zhang2@uncg.edu |
lab head | |
JongminWoo |
contact affiliation | The University of North Carolina at Greensboro |
contact email | jongminw@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034143
- Label: PRIDE project
- Name: Proteomic profiling of mouse Paneth cells microdissected from small intestinal tissue