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PXD034138-1

PXD034138 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGeneration of an Escherichia coli strain growing on methanol as sole energy and carbon source via the ribulose monophosphate cycle
DescriptionThe increasing demand for non-food competitive carbon sources such as methanol for biotechnology has brought methanol-utilizing bacteria, so-called methylotrophs, to focus. The product spectrum of natural methylotrophs and their genetic accessibility is limited and as an alternative approach, the introduction of methylotrophic metabolism into a biotechnologically well-established organism, such as Escherichia coli, represents a promising concept. By performing long-term evolution over 600 days, we obtained an E. coli strain that is able to grow on methanol as its sole carbon source at rates comparable to natural methylotrophic organisms. We confirmed that the strain forms its entire biomass from methanol. Furthermore, we sequenced the genome of the evolved strain and compared it to the genome of its ancestor. Intriguingly, we found several hundreds of mutations targeting genes of various functions, such as catalysis and regulation. Like the comparison of the genome before and after evolution, the investigation of the proteome would be of high interest. Proteomics would reveal the consequences of the regulatory mutations found in the genome and provide an overall picture of the adaptations by the cell enabling it to grow on methanol. The increasing demand for non-food competitive carbon sources such as methanol for biotechnology has brought methanol-utilizing bacteria, so-called methylotrophs, to focus. The product spectrum of natural methylotrophs and their genetic accessibility is limited and as an alternative approach, the introduction of methylotrophic metabolism into a biotechnologically well-established organism, such as Escherichia coli, represents a promising concept. By performing long-term evolution over 600 days, we obtained an E. coli strain that is able to grow on methanol as its sole carbon source at rates comparable to natural methylotrophic organisms. We confirmed that the strain forms its entire biomass from methanol. Furthermore, we sequenced the genome of the evolved strain and compared it to the genome of its ancestor. Intriguingly, we found several hundreds of mutations targeting genes of various functions, such as catalysis and regulation. Like the comparison of the genome before and after evolution, the investigation of the proteome would be of high interest. Proteomics would reveal the consequences of the regulatory mutations found in the genome and provide an overall picture of the adaptations by the cell enabling it to grow on methanol.
HostingRepositoryPRIDE
AnnounceDate2022-08-05
AnnouncementXMLSubmission_2022-08-05_15:08:25.087.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD034138
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLucas Hemmerle
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-26 01:19:13ID requested
12022-08-05 15:08:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Synthetic Methylotrophy, E.coli, Proteomics
Contact List
Julia A. Vorholt
contact affiliationDepartment of Biology, Institute of Microbiology, ETH Zürich, Switzerland
contact emailjvorholt@ethz.ch
lab head
Lucas Hemmerle
contact affiliationETH Zürich
contact emailhelucas@ethz.ch
dataset submitter
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Dataset FTP location
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