PXD034015 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative whole cell proteomics data of Vibrio cholerae O1 biovar El Tor str. N16961 versus full length cgtA knockdown and CgtA(∆CTD) during nutrient limiting condition |
Description | Adapting to nutritional downshifts is crucial for the survival of Vibrio cholerae. CgtA, a 50S ribosome-associated essential GTPase, is a bonafide stringent response protein. CgtA, in association with SpoT, modulates the intracellular (p)ppGpp alarmone levels in a nutrient-rich environment. We studied the influence of CgtA during the growth of V. cholerae in a minimal medium in contrast to its pre-defined role in a nutrient-rich medium. Here, we show the pleiotropic effects of CgtA on growth, viability, motility, morphology, and persister phenotype of a V. cholerae strain where the full-length wildtype (Wt) cgtA was deleted. An in-frame deletion of the 52 amino acids long unstructured C-terminal domain of the CgtA GTPase defined its in vivo functionality. Proteomic analyses revealed that loss of CgtA significantly altered 311 proteins involved in diverse cellular processes. However, the CgtA C-terminus deleted strain altered 240 proteins with a major overlap with the full-length cgtA deleted condition. A sustained mRNA expression pattern of CgtA is observed in a minimal medium. Whereas, in nutrient-rich LB medium, intermittent expression of cgtA is observed with the highest expression during the late-logarithmic to early-stationary phase. We propose that minimal media-associated nutrient stress coupled to cgtA depletion aggravates the intracellular stress in V. cholerae, leading to abnormal protein synthesis, altered DNA replication, and transcriptional machinery, negatively affecting cellular energy metabolism and many vital processes. This study suggests that the nutrient-media-dependent controlled expression of CgtA is a mechanism by which V. cholerae can remodel its transcriptome and proteome. Our study reveals an alternative facet of the survival of V. cholerae during nutritional downshift and the consequences concomitantly with cgtA knockdown as evident from the complex altered regulatory network. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-03 |
AnnouncementXML | Submission_2023-03-03_07:26:21.441.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Partha PratimDatta |
SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961); NCBI TaxID: 243277; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-20 10:43:44 | ID requested | |
⏵ 1 | 2023-03-03 07:26:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: CgtA GTPase, C-terminal domain CgtA truncation, nutrient limitation,Vibrio cholerae, ribosome, pleiotropic effects, cgtA knockdown |
Contact List
Dr. Partha PratimDatta |
contact affiliation | Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Kolkata, Mohanpur, District: Nadia, West Bengal, Pin Code: 741246, India |
contact email | partha_datta@iiserkol.ac.in |
lab head | |
Partha PratimDatta |
contact affiliation | Indian Institute of Science Education and Research (IISER), Kolkata |
contact email | partha_datta@iiserkol.ac.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034015
- Label: PRIDE project
- Name: Comparative whole cell proteomics data of Vibrio cholerae O1 biovar El Tor str. N16961 versus full length cgtA knockdown and CgtA(∆CTD) during nutrient limiting condition