PXD033998

PXD033998 is an original dataset announced via ProteomeXchange.

Title	Mice bone marrow-derived dendritic cells (BMDC) infected by Listeria: extracellular vesicles (EVs) proteome and PTMs
Description	In this study, we aimed to determine the role of EVs derived from Lm-infected DCs in the innate response, and the potential role of HDAC6 in DEV loading under these conditions. Our results show that Lm infection induced an elevated production of DEVs. Lm infection also altered protein sorting. Interestingly, HDAC6 deletion altered DEV protein sorting, although it did not affect acetylation of DEV-loaded proteins. However, protein ubiquitination was severely altered. Here, we show that the protein content of dendritic cells (DCs) and their secreted EVs (DEVs) vary dramatically during Lm infection. The proteomic profiles of DCs and DEV differ depending on whether HDAC6 is expressed or not.
HostingRepository	PRIDE
AnnounceDate	2022-08-30
AnnouncementXML	Submission_2022-08-30_02:42:14.807.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inmaculada Jorge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitinylated lysine; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-19 03:34:16	ID requested
⏵ 1	2022-08-30 02:42:15	announced

10.3389/FIMMU.2022.946358;

submitter keyword: Extracellular vesicles from dendritic cells, HDAC6-KO, multiplexed quantitative proteomics, ubiquitinilation, acetylation

Jesús María Vázquez Cobos
contact affiliation	Centron Nacional de Investigaciones Cardiovasculares, CNIC (Masdrid, Spain).
contact email	jvazquez@cnic.es
lab head
Inmaculada Jorge
contact affiliation	Vascular Biology and Inflammation
contact email	ijorge@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033998

PRIDE project URI

• PRIDE
  1. PXD033998
     1. Label: PRIDE project
     2. Name: Mice bone marrow-derived dendritic cells (BMDC) infected by Listeria: extracellular vesicles (EVs) proteome and PTMs