PXD033867 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome profiling of whole plasma and plasma-derived extracellular vesicles facilitates the detection of tissue biomarkers in the non-obese diabetic (NOD) mouse |
Description | Using plasma samples collected from pre-diabetic non-obese diabetic (NOD) mice (an experimental model of T1D) we performed parallel analysis of whole plasma samples and plasma-derived extracellular vesicle (EV) fractions using mass spectrometry (MS). Whole plasma samples were depleted of abundant proteins (albumin, transferrin and IgG) and subjected to high resolution isoelectric focusing (HiRIEF) LC-MS and relative quantification by TMT 10-plex. EV-enriched samples were analyzed by standard LC-MS/MS. Our results suggest that parallel profiling of EV-enriched plasma fractions and whole plasma samples increases the overall proteome depth and facilitates the discovery of tissue-enriched proteins in plasma. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:30:21.601.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xiaofang Cao |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 09:31:33 | ID requested | |
1 | 2022-10-20 02:11:35 | announced | |
⏵ 2 | 2023-11-14 08:30:23 | announced | 2023-11-14: Updated project metadata. |
Publication List
Diaz Lozano IM, Sork H, Stone VM, Eldh M, Cao X, Pernemalm M, Gabrielsson S, Flodstr, ö, m-Tullberg M, Proteome profiling of whole plasma and plasma-derived extracellular vesicles facilitates the detection of tissue biomarkers in the non-obese diabetic mouse. Front Endocrinol (Lausanne), 13():971313(2022) [pubmed] |
Keyword List
submitter keyword: Biomarkers |
extracellular vesicles |
exosomes |
mass spectrometry |
mouse |
non-obese diabetic (NOD) mouse |
plasma |
type 1 diabetes |
Contact List
Janne Lehtiö |
contact affiliation | Cancer Proteomics Mass Spectrometry, Oncology-Pathology Department,Karolinska Institutet |
contact email | janne.lehtio@ki.se |
lab head | |
Xiaofang Cao |
contact affiliation | Karolinska Institutet |
contact email | xiaofang.cao@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033867
- Label: PRIDE project
- Name: Proteome profiling of whole plasma and plasma-derived extracellular vesicles facilitates the detection of tissue biomarkers in the non-obese diabetic (NOD) mouse