PXD033857 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of four translation initiation proteins, EIF4E3, EIF4E4, EIF4G3 and EIF4G4 in Trypanosoma brucei |
Description | Gene expression in pathogenic protozoans of the family Trypanosomatidae has several novel features, for example in protein synthesis there are multiple eIF4F-like complexes. The eukaryotic eIF4F complex, formed by the eIF4E, eIF4G and eIF4A subunits, is responsible for the canonical selection of mRNAs required for the initiation of mRNA translation. The best-known complexes implicated in translation in trypanosomatids are based on two related pairs of eIF4E and eIF4G subunits (EIF4E3/EIF4G4 and EIF4E4/EIF4G3), whose functional distinctions have yet to be properly identified. Here, to define the interactome associated with both complexes in Trypanosoma brucei procyclic forms, we performed parallel immunoprecipitation experiments followed by identification of proteins co-precipitated with the two sets of tagged eIF4E and eIF4G subunits. A number of different protein partners, including RBPs and RNA helicases, were found to specifically co-precipitate with each complex. Highlights with the EIF4E4/EIF4G3 pair include RBP23, PABP1, EIF4AI and the CRK1 kinase. Co-precipitated partners with the EIF4E3/EIF4G4 pair are more diverse and include DRBD2, PABP2 and different zinc-finger proteins and RNA helicases.EIF4E3/EIF4G4 are known to be essential for viability and to better define their role. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-10 |
AnnouncementXML | Submission_2023-03-10_11:01:45.891.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MARIABEZERRA |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 08:56:28 | ID requested | |
⏵ 1 | 2023-03-10 11:01:47 | announced | |
2 | 2023-11-14 08:29:03 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bezerra MJR, Moura DMN, Freire ER, Holetz FB, Reis CRS, Monteiro TTS, Pinto ARS, Zhang N, Rezende AM, Pereira-Neves A, Figueiredo RCBQ, Clayton C, Field MC, Carrington M, de Melo Neto OP, . Front Mol Biosci, 9():971811(2022) [pubmed] |
Keyword List
submitter keyword: Trypanosoma brucei |
EIF4E |
EIF4G |
Contact List
Osvaldo PompĂlio de MeloNeto |
contact affiliation | Microbiology Department, IAM-Fiocruz, Pernambuco-Brasil |
contact email | osvaldo.pompiio@fiocruz.br |
lab head | |
MARIABEZERRA |
contact affiliation | Fiocruz Pernambuco |
contact email | maria.ribezerra@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033857
- Label: PRIDE project
- Name: Mass spectrometry analysis of four translation initiation proteins, EIF4E3, EIF4E4, EIF4G3 and EIF4G4 in Trypanosoma brucei