PXD033857

PXD033857 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of four translation initiation proteins, EIF4E3, EIF4E4, EIF4G3 and EIF4G4 in Trypanosoma brucei
Description	Gene expression in pathogenic protozoans of the family Trypanosomatidae has several novel features, for example in protein synthesis there are multiple eIF4F-like complexes. The eukaryotic eIF4F complex, formed by the eIF4E, eIF4G and eIF4A subunits, is responsible for the canonical selection of mRNAs required for the initiation of mRNA translation. The best-known complexes implicated in translation in trypanosomatids are based on two related pairs of eIF4E and eIF4G subunits (EIF4E3/EIF4G4 and EIF4E4/EIF4G3), whose functional distinctions have yet to be properly identified. Here, to define the interactome associated with both complexes in Trypanosoma brucei procyclic forms, we performed parallel immunoprecipitation experiments followed by identification of proteins co-precipitated with the two sets of tagged eIF4E and eIF4G subunits. A number of different protein partners, including RBPs and RNA helicases, were found to specifically co-precipitate with each complex. Highlights with the EIF4E4/EIF4G3 pair include RBP23, PABP1, EIF4AI and the CRK1 kinase. Co-precipitated partners with the EIF4E3/EIF4G4 pair are more diverse and include DRBD2, PABP2 and different zinc-finger proteins and RNA helicases.EIF4E3/EIF4G4 are known to be essential for viability and to better define their role.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:45.301.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIA BEZERRA
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 08:56:28	ID requested
1	2023-03-10 11:01:47	announced
⏵ 2	2023-11-14 08:29:03	announced	2023-11-14: Updated project metadata.

Bezerra MJR, Moura DMN, Freire ER, Holetz FB, Reis CRS, Monteiro TTS, Pinto ARS, Zhang N, Rezende AM, Pereira-Neves A, Figueiredo RCBQ, Clayton C, Field MC, Carrington M, de Melo Neto OP, . Front Mol Biosci, 9():971811(2022) [pubmed]

submitter keyword: Trypanosoma brucei

EIF4E

EIF4G

Osvaldo Pompílio de Melo Neto
contact affiliation	Microbiology Department, IAM-Fiocruz, Pernambuco-Brasil
contact email	osvaldo.pompiio@fiocruz.br
lab head
MARIA BEZERRA
contact affiliation	Fiocruz Pernambuco
contact email	maria.ribezerra@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033857

PRIDE project URI

• PRIDE
  1. PXD033857
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of four translation initiation proteins, EIF4E3, EIF4E4, EIF4G3 and EIF4G4 in Trypanosoma brucei