<<< Full experiment listing

PXD033829-1

PXD033829 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of the potential regulators of G6PD activity in different cell cycle
DescriptionFlag-G6PD was expressed in HeLa cells. Then the HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. These interphase cells were harvested. After a release of 8.5 h in fresh medium, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis.
HostingRepositoryPRIDE
AnnounceDate2023-05-18
AnnouncementXMLSubmission_2023-05-17_23:55:01.406.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAoxingCheng
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-12 02:19:51ID requested
12023-05-17 23:55:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: G6PD, Cell cycle
Contact List
zhenyeyang
contact affiliationUniversity of Science and technology of China
contact emailzhenye@ustc.edu.cn
lab head
AoxingCheng
contact affiliationuniversity of science and technology of China
contact emailchengax@mail.ustc.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033829
PRIDE project URI
Repository Record List
[ + ]