PXD033829

PXD033829 is an original dataset announced via ProteomeXchange.

Title	Identification of the potential regulators of G6PD activity in different cell cycle
Description	Flag-G6PD was expressed in HeLa cells. Then the HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. These interphase cells were harvested. After a release of 8.5 h in fresh medium, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2023-05-18
AnnouncementXML	Submission_2023-05-17_23:55:01.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AoxingCheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 02:19:51	ID requested
⏵ 1	2023-05-17 23:55:01	announced

Dataset with its publication pending

submitter keyword: G6PD, Cell cycle

zhenyeyang
contact affiliation	University of Science and technology of China
contact email	zhenye@ustc.edu.cn
lab head
AoxingCheng
contact affiliation	university of science and technology of China
contact email	chengax@mail.ustc.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033829

PRIDE project URI

• PRIDE
  1. PXD033829
     1. Label: PRIDE project
     2. Name: Identification of the potential regulators of G6PD activity in different cell cycle