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PXD033640-2

PXD033640 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePlasmid manipulation of bacterial behaviour through translational regulatory crosstalk
DescriptionpQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 plasmid free cells with cells carrying either WT pQBR103 or pQBR103 lacking rsmQ. Using comparative quantitative proteomics and RNAseq we showed that the loss of rsmQ leads to widespread proteomic changes in the absence of changes in mRNA abundance, suggesting RsmQ is a translational regulator of its host chromosome.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:25.063.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGerhard Saalbach
SpeciesList scientific name: Pseudomonas fluorescens (strain SBW25); NCBI TaxID: 216595; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-04 14:39:56ID requested
12023-01-13 03:39:14announced
22023-11-14 08:56:25announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TMT, RNAseq, translational regulator,Pseudomonas fluorescens SBW25, quantitative proteomics, plasmid
Contact List
Jacob G Malone
contact affiliationDept. Molecular Microbiology John Innes Centre Norwich Research Park Norwich, UK
contact emailjacob.malone@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliationBiological Chemistry
contact emailgerhard.saalbach@jic.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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