PXD033629 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MiPRISMA: Interactome profiling of small peptides and young microproteins translated from human short open reading frames Part2 |
Description | All species continuously evolve small open reading frames (sORFs) that can be templated for protein synthesis and may provide raw material for evolutionary adaptation. We analyzed the evolutionary origins of 7,264 recently cataloged human sORFs and found that most were evolutionary young and emerged de novo. We additionally discovered 221 yet uncataloged peptides smaller than 16 amino acids, for which we found evidence of translation in human and rodent tissues. To investigate the potential bioactivity of microproteins and peptides translated from young and very small ORFs we established MiPRISMA: a mass spectrometry-based interactome screen with sequence motif resolution. We assessed 266 candidates and implicated several in essential cellular processes including splicing, translational regulation, and endocytosis, a subset of which we validate in cellular assays. MiPRISMA provides a scalable platform to characterize small and evolutionarily young proteins, shedding light on a largely unexplored territory of the putative human proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-18 |
AnnouncementXML | Submission_2023-02-17_16:12:55.651.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MarieluiseKirchner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-04 14:24:01 | ID requested | |
⏵ 1 | 2023-02-17 16:12:56 | announced | |
2 | 2023-11-14 08:56:52 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: microproteins,proteomics, human, interactome |
Contact List
PhilippMertins |
contact affiliation | Berlin Institute of Health at Charite, Universitaetsmedizin Berlin, Core Facility Proteomics; Max Delbrueck Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany |
contact email | philipp.mertins@mdc-berlin.de |
lab head | |
MarieluiseKirchner |
contact affiliation | Proteomics Platform, BIH@Charite |
contact email | marieluise.kirchner@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033629
- Label: PRIDE project
- Name: MiPRISMA: Interactome profiling of small peptides and young microproteins translated from human short open reading frames Part2