PXD033608-1

PXD033608 is an original dataset announced via ProteomeXchange.

Title	Dnaaf4 and Dnaaf6 are required for assembly of ODAs and a subset of IDAs.
Description	Axonemal dynein motors are large multisubunit complexes that drive ciliary movement. Cytoplasmic assembly of these motor complexes involves several co-chaperones, some of which are related to the R2TP co-chaperone complex. The different roles of these co-chaperones is not completely known. Two such dynein assembly factors (DNAAFs) that are thought to function together in an R2TP-like complex are DNAAF4 (DYX1C1) and DNAAF6 (PIH1D3). Here we investigate the Drosophila homologues, CG14921/Dnaaf4 and CG5048/Dnaaf6. Surprisingly, Drosophila Dnaaf4 is truncated such it lacks a TPR domain, which is proposed to recruit HSP90 in human DNAAF4. Despite this, we provide evidence that Dnaaf4 and Dnaaf6 proteins can associate in an R2TP-like complex, and show functional conservation. Both are specifically expressed and required in the development of the two Drosophila cell types with motile cilia: mechanosensory chordotonal neurons and sperm. Flies that lack either gene are viable but with impaired chordotonal neuron function and lack motile sperm. We provide evidence that Dnaaf4 and Dnaaf6 are required for assembly of ODAs and a subset of IDAs.
HostingRepository	PRIDE
AnnounceDate	2022-06-23
AnnouncementXML	Submission_2022-06-23_03:57:23.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 09:55:04	ID requested
⏵ 1	2022-06-23 03:57:23	announced

Dataset with its publication pending

submitter keyword: Drosphila, Cilia

alex von kriegsheim
contact affiliation	University of Edinburgh
contact email	avonkri@ed.ac.uk
lab head
Alex von kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033608
     1. Label: PRIDE project
     2. Name: Dnaaf4 and Dnaaf6 are required for assembly of ODAs and a subset of IDAs.