<<< Full experiment listing


PXD033608 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDnaaf4 and Dnaaf6 are required for assembly of ODAs and a subset of IDAs.
DescriptionAxonemal dynein motors are large multisubunit complexes that drive ciliary movement. Cytoplasmic assembly of these motor complexes involves several co-chaperones, some of which are related to the R2TP co-chaperone complex. The different roles of these co-chaperones is not completely known. Two such dynein assembly factors (DNAAFs) that are thought to function together in an R2TP-like complex are DNAAF4 (DYX1C1) and DNAAF6 (PIH1D3). Here we investigate the Drosophila homologues, CG14921/Dnaaf4 and CG5048/Dnaaf6. Surprisingly, Drosophila Dnaaf4 is truncated such it lacks a TPR domain, which is proposed to recruit HSP90 in human DNAAF4. Despite this, we provide evidence that Dnaaf4 and Dnaaf6 proteins can associate in an R2TP-like complex, and show functional conservation. Both are specifically expressed and required in the development of the two Drosophila cell types with motile cilia: mechanosensory chordotonal neurons and sperm. Flies that lack either gene are viable but with impaired chordotonal neuron function and lack motile sperm. We provide evidence that Dnaaf4 and Dnaaf6 are required for assembly of ODAs and a subset of IDAs.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlex von kriegsheim
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-04 09:55:04ID requested
12022-06-23 03:57:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Drosphila, Cilia
Contact List
alex von kriegsheim
contact affiliationUniversity of Edinburgh
contact emailavonkri@ed.ac.uk
lab head
Alex von kriegsheim
contact affiliationUniversity of Edinburgh
contact emailalex.vonkriegsheim@ucd.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033608
PRIDE project URI
Repository Record List
[ + ]