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PXD033583-1

PXD033583 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids
DescriptionRhizobia are gram-negative bacteria able to establish a symbiotic interaction with leguminous plants. Due to their nitrogen fixing capacity, the study of these microorganisms has acquired great relevance for the agriculture. Rhizobia usually harbor many plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pLPU83a from Rhizobium favelukesii LPU83, a plasmid that showed a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory mechanism, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms play the same function. These findings set up a cornerstone for a new molecular circuit of conjugative transfer of plasmids.
HostingRepositoryPRIDE
AnnounceDate2022-08-29
AnnouncementXMLSubmission_2022-08-29_05:53:08.932.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGonzalo Torres Tejerizo
SpeciesList scientific name: Rhizobium favelukesii; NCBI TaxID: 348824;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-04 09:10:32ID requested
12022-08-29 05:53:09announced
22023-11-14 08:56:23announced2023-11-14: Updated project metadata.
Publication List
10.1128/MBIO.01949-22;
Keyword List
submitter keyword: Rhizobia, plasmid, conjugation
Contact List
Gonzalo Torres Tejerizo
contact affiliationInstituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata Argentina
contact emailgatt@biol.unlp.edu.ar
lab head
Gonzalo Torres Tejerizo
contact affiliationInstituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata
contact emailgatt@biol.unlp.edu.ar
dataset submitter
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