PXD033502

PXD033502 is an original dataset announced via ProteomeXchange.

Title	Taxon-specific toxicity and composition of venom produced by araneophagous white-tailed spiders (Lamponidae: Lampona sp.)
Description	Prey-specialised spiders are adapted to capture specific prey items, including dangerous prey such as ants, termites or other spiders. It has been observed that the venoms of specialists are often prey-specific and less complex than those of generalists, but venom composition has not been studied in detail in prey-specialised spiders. Here, we investigated the venom of the prey-specialised white-tailed spider (Lamponidae: Lampona sp.), which utilises specialised morphological and behavioural adaptations to capture spider prey. We hypothesised Lampona spiders also possess venomic adaptations, specifically, its venom is more effective to focal spider prey due to the presence of prey-specific toxins. We analysed the venom composition using proteo-transcriptomics and taxon-specific toxicity using venom bioassays. Our analysis identified 208 putative toxin sequences, comprising 103 peptides <10 kDa and 105 proteins >10 kDa. Most peptides belonged to one of two families characterised by scaffolds containing eight or ten cysteine residues. Protein toxins showed similarity to galectins, leucine-rich repeat proteins, trypsins and neprilysins. The venom of Lampona was shown to be spider-specific, as it was more potent against the preferred spider prey than against alternative prey represented by a cricket. In contrast, the venom of a related generalist (Gnaphosidae: Gnaphosa sp.) was similarly potent against both prey types. Prey-specific Lampona toxins were found to form part of the protein (>10 kDa) fraction of the venom. These data provide insights into the molecular adaptations of venoms produced by prey-specialised spiders.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:35.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Walker
SpeciesList	scientific name: Lampona murina; NCBI TaxID: 1908323;
ModificationList	amidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:01:25	ID requested
1	2023-02-07 16:30:10	announced
⏵ 2	2023-11-14 08:29:39	announced	2023-11-14: Updated project metadata.

Mich, á, lek O, Walker AA, Š, edo O, Zdr, á, hal Z, King GF, Pek, á, r S, ). Sci Rep, 12(1):21597(2022) [pubmed]

submitter keyword: venom

peptide toxin

spider

Glenn F. King
contact affiliation	Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia
contact email	glenn.king@imb.uq.edu.au
lab head
Andrew Walker
contact affiliation	University of Queensland
contact email	a.walker@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033502
     1. Label: PRIDE project
     2. Name: Taxon-specific toxicity and composition of venom produced by araneophagous white-tailed spiders (Lamponidae: Lampona sp.)