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PXD033459-1

PXD033459 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNative SEC-MS of the human HT29 colon adenocarcinoma cell line treated with the HSP90 inhibitor tanespimycin (17-AAG)
DescriptionThe molecular chaperone heat shock protein 90 (HSP90) works in concert with its co-chaperones to stabilize its client proteins, which include several drivers of oncogenesis and malignant progression. Pharmacologic inhibitors of HSP90 are proposed to trigger widespread remodeling of cellular protein complexes, including dissociation of co-chaperones from HSP90, disruption of client protein signaling networks, and recruitment of the protein ubiquitination and degradation machinery. However, proteomic studies to date have focused on inhibitor-induced changes in total protein levels, often overlooking protein complex alterations. Here, we use size-exclusion chromatography in combination with mass spectrometry (SEC-MS) to characterize the changes in native protein complexes following treatment with the HSP90 inhibitor 17-AAG in the human HT29 colon adenocarcinoma cell line.
HostingRepositoryPRIDE
AnnounceDate2022-12-20
AnnouncementXMLSubmission_2022-12-20_13:45:39.775.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRahulSamant
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-26 16:26:04ID requested
12022-12-20 13:45:40announced
22023-11-14 08:56:26announced2023-11-14: Updated project metadata.
22023-11-14 08:56:26announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Human, LC-MSMS, Colon, CoFrac-MS, HSP90, Adenocarcinoma, Cancer, SEC-MS, Colorectal, Pharmacology, Protein Complexes
Contact List
Rahul S.Samant
contact affiliationSignalling Programme, The Babraham Institute, Cambridge CB22 3AT, UK
contact emailrahul.samant@babraham.ac.uk
lab head
RahulSamant
contact affiliationBabraham Institute
contact emailrahul.samant@babraham.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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