PXD033459

PXD033459 is an original dataset announced via ProteomeXchange.

Title	Native SEC-MS of the human HT29 colon adenocarcinoma cell line treated with the HSP90 inhibitor tanespimycin (17-AAG)
Description	The molecular chaperone heat shock protein 90 (HSP90) works in concert with its co-chaperones to stabilize its client proteins, which include several drivers of oncogenesis and malignant progression. Pharmacologic inhibitors of HSP90 are proposed to trigger widespread remodeling of cellular protein complexes, including dissociation of co-chaperones from HSP90, disruption of client protein signaling networks, and recruitment of the protein ubiquitination and degradation machinery. However, proteomic studies to date have focused on inhibitor-induced changes in total protein levels, often overlooking protein complex alterations. Here, we use size-exclusion chromatography in combination with mass spectrometry (SEC-MS) to characterize the changes in native protein complexes following treatment with the HSP90 inhibitor 17-AAG in the human HT29 colon adenocarcinoma cell line.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:25.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rahul Samant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-26 16:26:04	ID requested
1	2022-12-20 13:45:40	announced
⏵ 2	2023-11-14 08:56:26	announced	2023-11-14: Updated project metadata.
⏵ 2	2023-11-14 08:56:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, LC-MSMS, Colon, CoFrac-MS, HSP90, Adenocarcinoma, Cancer, SEC-MS, Colorectal, Pharmacology, Protein Complexes

Rahul S. Samant
contact affiliation	Signalling Programme, The Babraham Institute, Cambridge CB22 3AT, UK
contact email	rahul.samant@babraham.ac.uk
lab head
Rahul Samant
contact affiliation	Babraham Institute
contact email	rahul.samant@babraham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD033459

PRIDE project URI

• PRIDE
  1. PXD033459
     1. Label: PRIDE project
     2. Name: Native SEC-MS of the human HT29 colon adenocarcinoma cell line treated with the HSP90 inhibitor tanespimycin (17-AAG)