PXD033391 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Determining kinome changes in response to Cytomegalovirus US28 expression and identifying US28 interacting proteins |
Description | Cytomegalovirus (CMV) reactivation of latent infection following immune dysregulation remains a serious risk for patients, often causing significant morbidity and mortality. Herein, we demonstrate the CMV-encoded GPCR, US28, in coordination with cellular Ephrin receptor A2 (EphA2), attenuates MAPK signaling, thereby limiting viral replication in latently-infected primary monocytes. To define the underlying mechanism by which US28 modulates MAPK signaling during latency, we used two unbiased approaches to: 1) profile kinome changes in response to US28 expression, and 2) define US28 interacting partners that could influence its function(s). First, we evaluated changes in the cellular kinome in response to US28 expression in THP-1 monocytic cells compared to control cells using multiplexed kinase inhibitor beads coupled with mass spectrometry (MIB-MS) to quantitatively measure kinase expression and abundance. Next, we leveraged proximity labeling technology, whereby we inserted the biotin ligase gene, birA, conjugated to a C-terminal hemagglutinin (HA) epitope tag in-frame with the US28 ORF to generate TB40/E-mCherry-US28-bioID-HA. We then infected fibroblasts with US28-bioID-HA, added biotin 18 h prior to harvesting the cells at 48 h post-infection (hpi), and analyzed the biotin-conjugated proteins following streptavidin capture by affinity purification-mass spectrometry (AP-MS). |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:36.491.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Herring |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-23 15:30:58 | ID requested | |
1 | 2023-03-11 03:58:04 | announced | |
⏵ 2 | 2023-11-14 08:42:44 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wass AB, Krishna BA, Herring LE, Gilbert TSK, Nukui M, Groves IJ, Dooley AL, Kulp KH, Matthews SM, Rotroff DM, Graves LM, O'Connor CM, Cytomegalovirus US28 regulates cellular EphA2 to maintain viral latency. Sci Adv, 8(43):eadd1168(2022) [pubmed] |
Keyword List
submitter keyword: viral infection,affinity purification-mass spectrometry, kinome |
Contact List
Christine O'Connor |
contact affiliation | Genomic Medicine, Infection Biology, Lerner Research Institute, Cleveland Clinic |
contact email | oconnoc6@ccf.org |
lab head | |
Laura Herring |
contact affiliation | UNC-Chapel Hill |
contact email | laura_herring@med.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033391 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033391
- Label: PRIDE project
- Name: Determining kinome changes in response to Cytomegalovirus US28 expression and identifying US28 interacting proteins